Journal: Molecular cancer research : MCR

Article Title: Caspase-9b drives cellular transformation, lung inflammation, and lung tumorigenesis

doi: 10.1158/1541-7786.MCR-21-0905

Figure Lengend Snippet: Macroscopic (A) and microscopic lung tumors observed in C9b mice over 21m old of age are summarized in B. *: One mouse developed a carcinoid tumor and adenocarcinoma. C. Histological analyses including H&E (a-c), Alcian Blue-PAS (d), and IHC staining with indicated antibodies (e-i) of lung tumors identified adenocarcinoma (a,d, g-i), squamous cell carcinoma (scc, b&e), and carcinoid tumors (c&f). Images are taken at 10x magnification. Inset in b is at 40x magnification. D&E. Gr1+ cells (D, IHC) in the lung per low power field (20x, 3 views per mouse) were counted in control (n=9) and C9b mice with (red, n= 5) or without (n=11) lung tumors (E). Data are means ± SEM. Adjusted p values are determined by ANOVA with Tukey’s multiple comparison test.

Article Snippet: Gomori Trichrome staining (Richard-Allan Scientific) and Alcian blue-PAS staining (Statlab) were performed following manufacturers’ instructions.

Techniques: Immunohistochemistry, Control, Comparison

Journal: Bioactive Materials

Article Title: Mesenchymal stem cell activity across a graded scaffold-hydrogel composite biomaterial for tendon-to-bone enthesis repair

doi: 10.1016/j.bioactmat.2025.07.017

Figure Lengend Snippet: Histological evaluation of triphasic scaffold structure and matrix remodeling. FFPE sections of hMSC-seeded triphasic scaffolds (n = 2) at both Day 1 and Day 21 were stained with either trichrome, alizarin red, or alcian blue staining. Representative images are provided of (A) a scan of a full scaffold (trichrome only), (B) the osseous region, (C) the tendinous region, and (D) the interfacial region.

Article Snippet: Samples were stained by one of four staining procedures: hematoxylin/eosin (H&E, SL200 & SL201, StatLab), alizarin red (Cat# 2003999, EMD Millipore Corp.), trichrome (Cat# ab150686, Abcam), or alcian blue (pH 2.5, Cat# KTABP2.5, StatLab) with staining procedures followed according to manufacturer recommendation.

Techniques: Staining

Journal: Molecular Medicine Reports

Article Title: Hypertrophic differentiation of mesenchymal stem cells is suppressed by xanthotoxin via the p38-MAPK/HDAC4 pathway

doi: 10.3892/mmr.2017.6886

Figure Lengend Snippet: Specific genes expression change during chondrogenic and hypertrophic differentiation. MSCs were induced by chondrogenic medium for 14 days and then treated with hypertrophic medium for another 14 days. (A) Relative mRNA expression levels of Sox9 and Col2a1 from MSCs in different groups (day 0 and 14). (B) Relative mRNA expression levels of Runx2 and Col10a1 from MSCs in different groups (day 14 and 28). XAT inhibited degradation of glycosaminoglycan and expressions of chondrocyte hypertrophic marker genes on mRNA level. (C) Representative alcian blue staining images of MSCs in different groups (day 28). (D) Quantification of mean intensity of alcian blue staining. (E) Relative mRNA expression levels of Col10a1, Runx2 and Mmp13 from MSCs in different groups (day 17, 21 and 28). The data in the figures represent the averages ± SD. Significant differences between the treatment and control groups are indicated in the graph with (*P<0.05) or (**P<0.01) or (***P<0.001).

Article Snippet: To demonstrate the deposition of cartilage matrix proteoglycans, representative cultures were collected at day 28, Then sulfated cartilage glycosaminoglycans (GAGs) were measured by alcian blue (ScienCell) staining.

Techniques: Expressing, Marker, Staining